Trigonella Foenum Gracum Seed Extract Inhibit Uptake of Glucose across Rat Everted Gut Sacs in Vitro

To investigate the absorption and the inhibition of glucose by the aqueous seed extracts of Trigonella foenum gracum. The small intestine of all animals were removed, washed with 0.9% (w/v) normal saline and intestinal segments were everted the empty sac was filled with 0.5 ml of the KHB buffer and placed in a stoppered conical flask with 5 ml of the buffer with varying concentration of glucose and 3.62 mf of Trigonella foenum gracum seed extract. The conical flask was placed in metabolic shaker at a frequency of 100-110 shakes/min. The incubation medium was bubbled for 1 min with a mixture of 95% O2 and 5% CO2. After 30 min of incubation medium the sacs were removed and the serosal fluid was drained into a test tube. Glucose in the mucosal and the serosal compartment was measured .Incubation of the Trigonella foenum gracum seed extracts with rat everted gut sacs resulted in the inhibition of the transport of glucose across the membrane. Based on the data obtained in this study, we can propose that aqueous seed extract of Trigonella foenum gracum may possess hypoglycemic properties that inhibit the glucose transport at the site of intestinal brush border membrane.